Analysis Note

ControlCells expressing the GST-tag.

Application

Research Sub CategoryEpitope Tags

Research CategoryEpitope Tags & General Use

Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate detects level of GST Tag & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunogen: GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH.

Specificity: Recognizes GST-fusion proteins.

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Immunogen

GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH. Clone DG122-2A7.

Legal Information

Kathon is a registered trademark of Rohm and Haas Co.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG in buffer containing (HRP) in 70% storage buffer PBS with 0.05% Kathon^® and 30% glycerol. Liquid at -20°C.

Quality

Routinely evaluated by western blot Analysis on recombinant GST protein.

Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.

Specificity

Recognizes GST-fusion proteins.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.